Bispecific antibodies combine specificities of two antibodies to simultaneously recognize/target different antigens or epitopes.
In our preferred constructs, the Fab part of the antibody molecule is reconfigured to single-chain variable fragment (scFv), so that the antibody molecule is presented as two “extended heavy chains” covalently associated by interchain disulfide bonds.
For each heavy chain, the N-terminal is scFv, the center part is CH2-CH3 domains of immunoglobulin γ1 chain, and at the C-terminal is a short peptide linker.
Here is an example of a bispecific antibody.
We validate the dual targeting ability of EGFR1/c-MET bispecific antibody (BsAb) by bridging ELISA and the results showing that anti-EGFR1/c-MET BsAb diaplays distinguished dual targeting ability, the EC50 values are shown below.
